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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is preparing a Gram stain of a mixed bacterial culture containing both Gram-positive and Gram-negative organisms. The correct order of reagents used in the Gram staining procedure is:

A) Crystal violet, iodine, decolorizer, safranin
B) Decolorizer, crystal violet, iodine, safranin
C) Safranin, crystal violet, decolorizer, iodine
D) Iodine, crystal violet, decolorizer, safranin

2. The gut microbiome has been implicated in the pathogenesis of autoimmune diseases. Dysbiosis, a disruption in the composition and function of the gut microbiota, can lead to increased intestinal permeability and the translocation of microbial products that trigger inflammatory responses. Which of the following bacterial components is a potent activator of the innate immune system and has been linked to the development of autoimmunity?

A) Peptidoglycan
B) Lipoteichoic acid (LTA)
C) Lipopolysaccharide (LPS)
D) Flagellin

3. A patient develops a localized skin infection after being bitten by a tick. The infection is characterized by a slowly expanding erythematous rash with central clearing (erythema migrans). The MOST likely causative agent is:

A) Rickettsia rickettsii
B) Ehrlichia chaffeensis
C) Borrelia burgdorferi
D) Anaplasma phagocytophilum

4. A patient with a history of travel to a rural area develops a febrile illness with myalgias and thrombocytopenia. Laboratory testing reveals the presence of morulae within circulating leukocytes. The MOST likely diagnosis is:

A) Lyme disease
B) Ehrlichiosis
C) Anaplasmosis
D) Babesiosis

5. A clinical microbiology laboratory is using a nucleic acid amplification test (NAAT) to detect Mycobacterium tuberculosis directly from sputum. A positive result indicates:

A) Drug susceptibility information for the M. tuberculosis strain.
B) The presence of viable M. tuberculosis organisms.
C) Active tuberculosis disease requiring immediate treatment.
D) The presence of M. tuberculosis DNA, which could be from viable or non-viable organisms.

Solutions: